Isolation Of Oligonucleotides By Scalable IEX-CPC
Pure oligonucleotide fractions with high yield? Ready.
Learn how centrifugal partition chromatography (CPC) achieves unparalleled purity and yield in a single step, promising industrial-scale production of oligonucleotides for oligonucleotide development crucial in vaccine development and pharmacology.
This study was conducted to purify nucleotides, specifically a 20-mer single-stranded unmodified oligonucleotide, initially possessing 88.5% purity. Employing CPC, a two-phase solvent system of ethyl acetate/n-butanol/nuclease-free water was utilized. This approach yielded a remarkable purity of 96.6% and a calculated yield of 94.5%. Furthermore, the method enabled removal of organic solvents through lyophilization, with residual salts and additives easily eliminated via alcohol precipitation, which resulted in a pure and ready-for-application material. Download now for insights.
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CPC Modeler
CPC Modeler is a desktop-sized CPC platform that offers a mg to g/cycle loading capacity and high purify. The platform comes with a CPC Simulator tool which lets you find the most effective CPC purification method and evaluate the productivity of your CoI, using an extended digital library, the partition coefficient, and the composition of your crude.
Validate your digital purification setup with the provided semi-preparative partition chromatographic equipment. Test your purification method on the benchtop CPC system, and fine-tune your operational parameters for optimal performance. See the results of the in-silico purification method in real life.
Continuous CPC
Continuous CPC Continuous CPC platform is a continuous, multiple dual-mode, dual-rotor CPC platform that fits the continuous manufacturing and industrial-scale production of compounds.
Unlike the standard batch operational mode, it enhances throughput with its continuous injection mode, allowing for more efficient sample processing and multiple increase in production, beside low operational cost. It’s highly scalable, adaptable to various sizes and requirements, and built with robustness in mind, meeting GMP industrial standards for durability. The device incorporates an on-demand solvent handling system that can be tailored to specific industrial standards.
About CPC
CPC is a liquid-liquid preparative chromatographic technique, in which separation occurs between two immiscible liquid solvent systems: acting as both the stationary and mobile phases. The liquid stationary phase is immobilized by a strong centrifugal force inside cells attached to a rotor, and the mobile phase is then pumped through the stationary phase in the form of tiny droplets. Separation of the injected sample is based on the sample component’s varying partition coefficients between the mobile and stationary phases.
CPC has several advantages like low operational expenses, easy scalability, elimination of sample loss and irreversible adsorption, operation without silica gel or any solid stationary phase, and the possibility to separate a wide array of compounds.
About RotaChrom
RotaChrom Technologies develops and manufactures industrial-scale centrifugal partition chromatography (CPC) technology platforms. Founded in 2014, RotaChrom distributes its CPC devices around the world. With the help of their industry-leading chemists and engineers, their instruments revolutionized purification by providing cost-effective industrial-scale chromatography solutions to customers in various industries, including pharmaceutical, biotechnology, chemistry, nutraceutical, and botanical extracts.
