Leverage the Applications of CPC
Enhance Your Purification With CPC Technology
Liquid liquid chromatography is a combination of liquid liquid separation principles combined with the methodology of chromatography. In this technique, separation occurs between two immiscible liquid phases, as both the mobile and stationary phases are liquids.
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To help you learn about CPC applications, we would like to offer you a free brochure which includes many centrifugal partition chromatography applications that our team of qualified experts developed throughout the years.
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Isolation of an Antibiotic Oligopeptide
- Active Pharmaceutical Ingredient (API): Cyclosporine A (CsA)
- Goal: Purification
Polishing of a steroid API
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API: Steroid
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Goal: Purification
A microbiological fermentation process produces this API. However, if reaction selectivity is compromised, stereoisomer of the API and other by-products appear in the process. Conventional production techniques of this API require steps that result in a significant loss of the yield. During the last step only, which is a preparative HPLC step, the loss is 70%. Using our CPC method, a yield of above 90% can be achieved with the similarly high purity. Because of the remarkable selectivity value, the system can be loaded well despite the low solubility of the API.
Ionizable LNP Lipid Purification by CPC
Lipid nanoparticles (LNPs) are novel powerful pharmaceutical drug delivery vehicles, intensively utilized in the formulation (i.e., encapsulation) of mRNA vaccines. These systems primarily rely on four lipid components: a PEG lipid, amino (cationic) lipid, structural lipid, and cholesterol [1]. The ionizable (cationic) lipids are key components in LNPs, since they can form aggregate complexes with nucleic acids through a charge-charge interaction (Fig. 1).
In an ongoing feasibility study an alkane/alcohol/water solvent system based multiple dual mode (MDM) CPC method was developed to isolate a cationic lipid with a proprietary structure from a synthetic reaction mixture (containing unreacted starting material and over-alkylated byproducts as major impurities). The CPC method outperformed the currently used normal-phase flash chromatography in terms of yield and circumvented the stability issue experienced on solid stationary phase column chromatography with dichlormethane-methanol solvents.
In summary, 320 mg of lipid crude was processed in 35 min to produce the target lipid in 93% yield and with 98% purity on a lab-scale CPC system (Fig. 2). The scale-up of this promising methodology to pilot-scale is currently in progress.
RotaChrom brings industrial-scale CPC solutions to businesses, researchers, CROs and CDMOs
What you have seen so far is just the tip of the iceberg.
If you'd like to dive deeper, please fill in the form above, and grab your free copy of RotaChrom's 'Advanced Chromatography' white paper. The paper covers 4 in-depth chapters about CPC and LLC.
RotaChrom Technologies developed the world’s first industrial scale centrifugal partition chromatography technology solution. These liquid chromatography machines revolutionized purification by providing innovative, sustainable, and efficient industrial scale LC-CPC chromatography to customers all over the world in various industries, including pharmaceutical, nutraceutical and botanical extracts.
RotaChrom’s proven GMP compliant CPC designs are effective solutions when looking for a high-purity, high-capacity, cost-effective and environmentally conscious solution. RotaChrom, with its in-house research laboratory employing world class PhD chemists, engineers and leading business professionals on multiple continents, is the best partner in purification.
Our portfolio includes several products, which can help you scale your business from research to industry. Select between lab scale, pilot scale or industrial scale purification:
- The rCPC platform for pilot-scale purification tasks within a laboratory environment. It is ideal for all batch sizes, smaller or larger, or campaign type of research work, and continuous-batch production.
- The iCPC platform, which is the largest commercially available centrifugal partition Chromatography instrument in the world. It is designed for industrial-scale purification projects in continuous batch operation mode.
